Pcr Process And Arrangement For Dna Amplification

专利名称：Pcr Process And Arrangement For Dna

Amplification Using Dry Reagents

发明人：Walter Gumbrecht,Peter Paulicka,Manfred

Stanzel

申请号：US11587567申请日：20050426

公开号：US20080241890A1公开日：20081002

专利附图：

摘要：A PCR process for DNA amplification with thermocyclisation of thecorresponding reagents is disclosed, in which total integration of all substances and

process steps is achieved in a closed, single-use unit (so-called cartridge) in which thereagents are stored in a storage-stable form at room temperature. According to theprocess, the water-soluble reagents are covered by a water-insoluble medium, then theDNA to be amplified is supplied and the water-insoluble medium is eliminated, so thatthe water-soluble reagents are dissolved and PCR can start. In the correspondingarrangement, a test unit designed as a single-use produce (a so-called cartridge) has atleast one micro-channel or micro-cavity for receiving a PCR reagent. The PCR reagents inthe form of a mixture which can be dried at a negligible vapour pressure and forms astorage-stable substance substance at room temperature adhere to the walls of themicro-channel or micro-cavity and form a thin film covered by an insoluble medium.

申请人：Walter Gumbrecht,Peter Paulicka,Manfred Stanzel

地址：Herzogenaurach DE,Erlangen DE,Erlangen DE

国籍：DE,DE,DE

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