pRetroX-TetOne逆病毒载体使用说明

编号 北京华越洋VECT55131 载体名称 pRetroX-TetOne

pRetroX-TetOne逆病毒载体基本信息：

pRetroX-TetOne 载体名称:

质粒类型: 逆病毒载体；四环素调控载体 高拷贝/低拷贝: 高拷贝 克隆方法: 限制性内切酶，多克隆位点

TRE3GS , hPGK 启动子:

8508 bp 载体大小:

-- 5' 测序引物及序列:

-- 3' 测序引物及序列:

-- 载体标签:

载体抗性: 氨苄青霉素

-- 筛选标记:

DH5α 克隆菌株:

宿主细胞（系）: 常规细胞系（293、CV-1、CHO等）

逆病毒载体pRetroX-TetOne是四环素调控系统载体；与其

备注: 它四环素诱导系统不同，pRetroX-TetOne集调控功能与应

答功能于一体。

稳定性: 稳表达 组成型/诱导型: 诱导型 病毒/非病毒: 逆转录病毒

pRetroX-TetOne载体质粒图谱和多克隆位点信息：

pRetroX-TetOne载体简介： pRetroX-TetOne载体序列：

ORIGIN

1 AAGCTAGCTT TGCTCTTAGG AGTTTCCTAA TACATCCCAA ACTCAAATAT ATAAAGCATT 61 TGACTTGTTC TATGCGCCTA GGAGCTTGGC CCATTGCATA CGTTGTATCC ATATCATAAT 121 ATGTACATTT ATATTGGCTC ATGTCCAACA TTACCGCCAT GTTGACATTG ATTATTGACT 181 AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC 241 GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG 301 ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA 361 TGGGTGGAGT ATTTACGCTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA 421 AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC 481 ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC 541 ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA 601 TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG 661 GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCAT CTAGAGCGCT 721 TCTGCTCCCC GAGCTCTATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG 781 ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC 841 TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG 901 GGTCTTTCAT TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC 961 ACCACCGGGA GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA 1021 TGACTGATTT TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA 1081 CCCGTGGTGG AACTGACGAG TTCGGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG 1141 ACTTCGGGGG CCGTTTTTGT GGCCCGACCT GAGTCCTAAA ATCCCGATCG TTTAGGACTC 1201 TTTGGTGCAC CCCCCTTAGA GGAGGGATAT GTGGTTCTGG TAGGAGACGA GAACCTAAAA 1261 CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGTTTGGG ACCGAAGCCG CGCCGCGCGT 1321 CTTGTCTGCT GCAGCATCGT TCTGTGTTGT CTCTGTCTGA CTGTGTTTCT GTATTTGTCT 1381 GAAAATGAGG GCCCGGGCTA GCCTGTTACC ACTCCCTTAA GTTTGACCTT AGGTCACTGG 1441 AAAGATGTCG AGCGGATCGC TCACAACCAG TCGGTAGATG TCAAGAAGAG ACGTTGGGTT 1501 ACCTTCTGCT CTGCAGAATG GCCAACCTTT AACGTCGGAT GGCCGCGAGA CGGCACCTTT 1561 AACCGAGACC TCATCACCCA GGTTAAGATC AAGGTCTTTT CACCTGGCCC GCATGGACAC 1621 CCAGACCAGG TGGGGTACAT CGTGACCTGG GAAGCCTTGG CTTTTGACCC CCCTCCCTGG 1681 GTCAAGCCCT TTGTACACCC TAAGCCTCCG CCTCCTCTTC CTCCATCCGC CCCGTCTCTC 1741 CCCCTTGAAC CTCCTCGTTC GACCCCGCCT CGATCCTCCC TTTATCCAGC CCTCACTCCT 1801 TCTCTAGGCG CCCCCATATG GCCATATGAG ATCCTATATG GGGCACCCCC GCCCCTTGTA 1861 AACTTCCCTG ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA 1921 CAGGCTCTCT ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA 1981 CAACTGGACC GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC 2041 CGACACCAGA CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC 2101 ACCCCCACCG CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG 2161 GCTGCCGACC CCGGGGGTGG ACCATCCTCT AGATCATAAT CAGCCATACC ACATTTGTAG 2221 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 2281 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAGGCAATA 2341 GCATCACAAA TTTCACAAAT AAGGCATTTT TTTCACTGCA TTCTAGTTTT GGTTTGTCCA 2401 AACTCATCAA TGTATCTTAT CATGTCTGGA TCTCAAATCC CTCGGAAGCT GCGCCTGTCT

2461 TAGGTTGGAG TGATACATTT TTATCACTTT TACCCGTCTT TGGATTAGGC AGTAGCTCTG 2521 ACGGCCCTCC TGTCTTAGGT TAGTGAAAAA TGTCACTCTC TTACCCGTCA TTGGCTGTCC 2581 AGCTTAGCTC GCAGGGGAGG TGGTCTGGAT CCGCCGGCAC CGGTGTATAC GGGAATTCTT 2641 TACGAGGGTA GGAAGTGGTA CGGAAAGTTG GTATAAGACA AAAGTGTTGT GGAATTGAAG 2701 TTTACTCAAA AAATCAGCAC TCTTTTATAG GCGCCCTGGT TTACATAAGC AAAGCTTATA 2761 CGTTCTCTAT CACTGATAGG GAGTAAACTG GATATACGTT CTCTATCACT GATAGGGAGT 2821 AAACTGTAGA TACGTTCTCT ATCACTGATA GGGAGTAAAC TGGTCATACG TTCTCTATCA 2881 CTGATAGGGA GTAAACTCCT TATACGTTCT CTATCACTGA TAGGGAGTAA AGTCTGCATA 2941 CGTTCTCTAT CACTGATAGG GAGTAAACTC TTCATACGTT CTCTATCACT GATAGGGAGT 3001 AAACTCGAGG TGATAATTCC ACGGGGTTGG GGTTGCGCCT TTTCCAAGGC AGCCCTGGGT 3061 TTGCGCAGGG ACGCGGCTGC TCTGGGCGTG GTTCCGGGAA ACGCAGCGGC GCCGACCCTG 3121 GGTCTCGCAC ATTCTTCACG TCCGTTCGCA GCGTCACCCG GATCTTCGCC GCTACCCTTG 3181 TGGGCCCCCC GGCGACGCTT CCTGCTCCGC CCCTAAGTCG GGAAGGTTCC TTGCGGTTCG 3241 CGGCGTGCCG GACGTGACAA ACGGAAGCCG CACGTCTCAC TAGTACCCTC GCAGACGGAC 3301 AGCGCCAGGG AGCAATGGCA GCGCGCCGAC CGCGATGGGC TGTGGCCAAT AGCGGCTGCT 3361 CAGCAGGGCG CGCCGAGAGC AGCGGCCGGG AAGGGGCGGT GCGGGAGGCG GGGTGTGGGG 3421 CGGTAGTGTG GGCCCTGTTC CTGCCCGCGC GGTGTTCCGC ATTCTGCAAG CCTCCGGAGC 3481 GCACGTCGGC AGTCGGCTCC CTCGTTGACC GAATCACCGA CCTCTCTCCC CAGGGGGATC 3541 ATCGAATTAC CATGTCTAGA CTGGACAAGA GCAAAGTCAT AAACTCTGCT CTGGAATTAC 3601 TCAATGGAGT CGGTATCGAA GGCCTGACGA CAAGGAAACT CGCTCAAAAG CTGGGAGTTG 3661 AGCAGCCTAC CCTGTACTGG CACGTGAAGA ACAAGCGGGC CCTGCTCGAT GCCCTGCCAA 3721 TCGAGATGCT GGACAGGCAT CATACCCACT CCTGCCCCCT GGAAGGCGAG TCATGGCAAG 3781 ACTTTCTGCG GAACAACGCC AAGTCATACC GCTGTGCTCT CCTCTCACAT CGCGACGGGG 3841 CTAAAGTGCA TCTCGGCACC CGCCCAACAG AGAAACAGTA CGAAACCCTG GAAAATCAGC 3901 TCGCGTTCCT GTGTCAGCAA GGCTTCTCCC TGGAGAACGC ACTGTACGCT CTGTCCGCCG 3961 TGGGCCACTT TACACTGGGC TGCGTATTGG AGGAACAGGA GCATCAAGTA GCAAAAGAGG 4021 AAAGAGAGAC ACCTACCACC GATTCTATGC CCCCACTTCT GAAACAAGCA ATTGAGCTGT 4081 TCGACCGGCA GGGAGCCGAA CCTGCCTTCC TTTTCGGCCT GGAACTAATC ATATGTGGCC 4141 TGGAGAAACA GCTAAAGTGC GAAAGCGGCG GGCCGACCGA CGCCCTTGAC GATTTTGACT 4201 TAGACATGCT CCCAGCCGAT GCCCTTGACG ACTTTGACCT TGATATGCTG CCTGCTGACG 4261 CTCTTGACGA TTTTGACCTT GACATGCTCC CCGGGTAACT AAGTAAGATC CTGTTAATCA 4321 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGATATT CTTAACTATG TTGCTCCTTT 4381 TACGCTGTGT GGATATGCTG CTTTAATGCC TCTGTATCAT GCTATTGCTT CCCGTACGGC 4441 TTTCGTTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 4501 CGTTGTCCGT CAACGTGGCG TGGTGTGCTC TGTGTTTGCT GACGCAACCC CCACTGGCTG 4561 GGGCATTGCC ACCACCTGTC AACTCCTTTC TGGGACTTTC GCTTTCCCCC TCCCGATCGC 4621 CACGGCAGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTA GGTTGCTGGG 4681 CACTGATAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCG ATTCCTGCGG AGATATCAGT 4741 GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG AAGCCTATAG AGTACGAGCC 4801 ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC 4861 CTGTAGGTTT GGCAAGCTAG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCAGACATCA 4921 ACAAAAGAGC CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG 4981 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 5041 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACGCGTATG

5101 CCGCATAGTT AAGCCACCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG 5161 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG 5221 CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG 5281 GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC 5341 CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC 5401 CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC 5461 GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC 5521 CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGGCT CCGACCGAAG CCGACCCGGG 5581 CGGCCCCGCC GACCCCGCAC CCGCCCCCGA GGCCCACCGA CTCTAGAGGA TCATAATCAG 5641 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA 5701 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG 5761 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 5821 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GGCGCGTCGA ATTAATTCAC 5881 TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC 5941 TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC 6001 CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA 6061 CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG 6121 CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT 6181 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC 6241 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGATGACGAA AGGGCCTCGT GATACGCCTA 6301 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 6361 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 6421 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 6481 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 6541 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 6601 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 6661 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 6721 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 6781 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 6841 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 6901 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 6961 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 7021 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 7081 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 7141 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 7201 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 7261 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 7321 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 7381 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 7441 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 7501 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 7561 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 7621 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 7681 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG

7741 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 7801 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 7861 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC 7921 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 7981 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 8041 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 8101 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 8161 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 8221 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 8281 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 8341 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 8401 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 8461 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCC //

pRetroX-TetOne其他相关逆病毒载体： pVSV-G pMKO.1-GFP pBABE-hygro-hTERT pBABE-GFP pBABE-Puro pVPack-Eco pCL-Eco pMSCV-PIG pFB-hrGFP pCFB pRetroX-TetOne pRetroX-PTuner-IRES pRetroX-Tet-On Advanced pRetroX-Tight-Pur pRetroQ-DsRed-Monomer-C1 pRetroX-SG2M-Cyan pMSCV-FLIP-puro-dsRed-GFP-miRNA pBABE-puro-IRES-EGFP pBABE-neo-hTERT pBABE-hygro pVPack-GP pVPack-Ampho pUMVC pQCXIN pFB-Luc pRetro-Lib pRetroX-PTuner pRetroX-TRE3G pRetroX-G1-Red pRetroQ-DsRed-Monomer-N1 pRetroX-IRES-DsRedExpress pLXSN pSuper.Retro.Puro pRetroX-IRES-ZsGreen1 pMSCVpuro

pCLXSN pBABE pBABE-neo-SV40LT pBABE-neo pVPack-10A1 pCMV-Gag-Pol pCMV-VSV-G pFB-ERV pFB-Neo pRetroX-Tight-Pur-Luc pRetroX-PTuner2 pRetroX-TRE3G-Luc pRetroQ-mCherry-N1 pRetroQ-AcGFP1-C1 RNAi-Ready pSIREN-RetroQ-DsRed-Express pQCXIX RNAi-Ready pSIREN-RetroQ-ZsGreen1 pMSCVhyg pSuper.Retro.Neo pMKO.1-puro pBABE-HAII pBABE-Puro SV40LT pBABE-zeo pVPack-VSV-G pCL-Ampho pCMV-VSVG pCFB-EGSH pFB pRetroX-TetOne-luc pRetroX-PTuner2-C pRetroX-Tet3G pRetroX-Tight-Hyg-Luc pRetroQ-mCherry-C1 pRetroQ-AcGFP1-N1 pLXIN pRetroX-Tet-Off Advanced pRetroX-Tight-Hyg pLNCX2 pSilencer5.1-U6 Retro RNAi-Ready pSIREN-RetroQ pQCXIH pSuper.Retro.Neo+GFP pRetroX-IRES-DsRed Express pMSCVneo pSilencer5.1-H1 Retro