IMPROVED β-FRUCTOFURANOSIDASE

专利名称：IMPROVED β-FRUCTOFURANOSIDASE发明人：TOCHIO Takumi,栃尾　巧,NAKAMURA Saki,中

村　早岐,YAHARA Misa,八原　美沙,FUJIITadashi,藤井　匡,TAMURA Keisuke,田村　圭輔

申请号：JP2014/084680申请日：20141226

公开号：WO2015/099166A1公开日：20150702

专利附图：

摘要：[Problem] To provide an improved β-fructofuranosidase which enables theproduction of kestose with high efficiency while reducing the amount of a by-product

such as nystose produced during the production of kestose. [Solution] An improved β-fructofuranosidase comprising an amino acid sequence which is produced by introducingan amino acid mutation into an amino acid sequence for a β-fructofuranosidase having60% or higher identity to the amino acid sequence for wild-type β-fructofuranosidasewhich is represented by SEQ ID NO: 2, wherein the amino acid mutation is such a mutationthat, when amino acid sequence alignment is performed, a histidine (H) residue

corresponding to the position-395 as numbered from the N-terminal of the amino acidsequence for the wild-type β-fructofuranosidase which is represented by SEQ ID NO: 2can be replaced by an arginine (R) residue or a lysine (K) residue.

申请人：B FOOD SCIENCE CO., LTD.,物産フードサイエンス株式会社,MICROBIOPHARMJAPAN CO., LTD.,日本マイクロバイオファーマ株式会社

地址：24-12, Kitahama-machi, Chita-shi, Aichi 4780046 JP,〒4780046 愛知県知多市北浜町２４番１２ Aichi JP,3-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1040031 JP,〒1040031 東京都中央区京橋一丁目３番１号 Tokyo JP

国籍：JP,JP,JP,JP

代理人：SAGAWA Shingo et al.,佐川　慎悟

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